Thursday, 4 January 2018

Herbal Therapy: Green Tea and Its Bioactive Polyphenols Epigallocatechin-3 Gallate (EGCG) in Attenuated Risk and Treatment of Hepatitis B

Kyle J. Norton 

Green tea may have a therapeutic and positive effect in reduced risk and treatment of hepatitis B, some scientists suggested.

Hepatitis B is a viral infection of acute and chronic liver disease, caused by a double stranded DNA virus HBV transmitted through infected blood, semen, or other body fluid.

According to the statistic, positive carriers among Canadian is estimated to be 0.5% to 1.0%., depending to ethnicity.

Green tea, a precious drink processes numbers of health benefit known to almost everyone in Asia and Western world. However, as yin in nature herbal medicine or food, long term injection of large amounts may obstruct the balance of yin-yang, induced "yin excessive syndrome" or "yang vacuity syndrome" including weaken immunity and painful case of GERD,... according to traditional Chinese medicine's Yin-Yang theory.

According to the evaluation of the effect of green tea bioactive polyphenos epigallocatechin-3 gallate (EGCG) and risk of hepatitis B, application of the phytochemical  inhibited HBV replication in the testing Hep3B2.1-7 hepatocellular carcinoma cell line, through a significant effect in reduced prevalence of liver damage caused by hepatitis B virus.

Interesting, injection of EGCG  at the dose of less than 100 μM in the observed liver cancer cell line showed no effect in induced cell apoptosis and decreased proliferation but exhibited a strong effect in ameliorated expression of surface antigen of the hepatitis B virus (HBsAg) and antigen of hepatitis B virus circulated in infected blood (HBeAg) levels found by the ELISA method and the polymerase chain reaction (PCR)assay.

Further differentiation in different HepG2 2.2.15 cells to compare the effective of green tea with standard antiretroviral medication lamivudine (3TC), researchers indicated that administration of EGCG shows a significant inhibition of viral proliferation through attenuated expression of levels of HBsAg and HBeAg in the supernatant and extracellular HBV DNA in a dose- and time-dependent manner. At doses of 50-200 μg/ml EGCG displayed strong effects in compared to lamivudine (3TC) at dose of 200 μg/ml.

More importantly, application of EGCG also suppressed the extracellular HBV DNA, a nutrient source for viral cell proliferation and survival through attachment, aggregation, and stabilization of microcolonies.

The results of the experiments pointed out that green tea EGCG consists a potential anti-HBV activity with low toxicity.

Additional investigation of green tea EGCG effect in the early stages of infection caused by four different Hepatitis B virus genotypes A and D also found that at dose of more than 80%, the phyochemical inhibited both DMSO-differentiated HuS-E/2 cells and HA-NTCP-expressing Huh7 cells involved cancer cell process in early stage of proliferation through induced protein degradation and ameliorated serum of tansferrin.(the iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids)which isconsidered as a liver fibrosis biomarker in patients with chronic hepatitis B.

However, Treatment of cells with EGCG had no effect on HBV genome replication or virion secretion and the characteristic of HBV virion and the expression of known HBV entry factors were unaltered out side of the host before attacking the liver tissue.

All these results suggested that the green tea-derived molecule EGCG potently inhibits HBV entry and could be used in prevention and treatment of Hepatitis B. Intake of green tea supplement should be taken with to prevent overdoses cause of acute liver toxicity.


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Author Biography
Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.


Sources
(1) (-)-Epigallocatechin-3-gallate inhibits entry of hepatitis B virus into hepatocytes by Huang HC1, Tao MH2, Hung TM3, Chen JC4, Lin ZJ5, Huang C6.(PubMed)
(2) Preventive effects of a major component of green tea, epigallocathechin-3-gallate, on hepatitis-B virus DNA replication by Karamese M1, Aydogdu S, Karamese SA, Altoparlak U, Gundogdu C.(PubMed)
(3) Green tea polyphenol, epigallocatechin-3-gallate, possesses the antiviral activity necessary to fight against the hepatitis B virus replication in vitro by Pang JY1, Zhao KJ, Wang JB, Ma ZJ, Xiao XH.(PubMed)

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