Herbal Therapy: Green Tea (-)-Epigallocatechin-3-O-Gallate (EGCG) For Prevention and Treatment of Melanoma

Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)

Green tea may have a profound and sustainable effect in reduced risk and treatment of melanoma, some scientists suggested.

Melanoma, is a medical condition characterized by irregular cell growth of  pigment-containing cells known as melanocytes. At the later stage, the cancerous cells may travel a distance away to infect other healthy organs and tissues.

According to the study, approximately 7,200 Canadians will be diagnosed with melanoma skin cancer.

Green tea, a precious drink processes numbers of health benefit known to almost everyone in Asia and Western world. However, as yin in nature herbal medicine or food, long term injection of large amounts may obstruct the balance of yin-yang, induced "yin excessive syndrome" or "yang vacuity syndrome" including weaken immunity and painful case of GERD,... according to traditional Chinese medicine's Yin-Yang theory.

In the review of literature published online, green tea bioactice EGCG significantly inhibited the growth, migration and invasion of melanoma cells through numbers of mechanism.

Gene implications in initiated cancer development and cancerous cells proliferation have gone through extensive research, particularly in melanoma.

In melanoma, green tea biactice EGCG demonstrated a significant effect in knockdown gene over expression (TRAF6) in reduced migration and invasion of melanoma.

TRAF6 is a protein with function in activation of IkappaB kinase (IKK) in response to inflammation.

In fact, green tea biactive EGCG directly bind to TRAF6 in melanoma in attenuated the interaction of
between TRAF6 and UBC13(E2) in activated the protein (IkappaB kinase (IKK)) in production of pro inflammatory cytokins.

The ubiquitin-conjugating enzyme(UBC13(E2)) are essential in activation protein (NF- kappa B) in controls transcription of DNA, cytokine production and cell survival.

Furthermore, the phytochemicals also suppressed the TRAF6 E3 ubiquitin ligase activity of the tumor generated by TRAF6 in regulation of important biological processes, particularly, in cell division and for the survival of the tumors.

Additionally, green tea biactice EGCG also regulated the gene expression of proteins (IκBα and p-TAK1 e) in modulation and inactivated NF-κB-directed gene expression of inflammation, immunity, cell proliferation, differentiation, and survival in melanoma.

Dr. Zhang J, the lead author said, " EGCG is a novel E3 ubiquitin ligase inhibitor that could be used to target TRAF6 for chemotherapy or the prevention of melanoma".

Other researchers in focus of Green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) effect in attenuated and treatment of melanoma in the gene implication via MicroRNAs (miRNAs, non-coding RNAs involved in various biological processes by regulating their target genes) also indicated that

Green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) inhibited melanoma tumor growth by activating 67-kDa laminin receptor (67LR) signaling which has an function in down regulated tumor cell proliferation and tumor formation by inducing apoptosis.

The bioactive compound also up-regulated miRNA-let-7b expression in induced tumor suppressor function in decrease function of 67LR in enhanced invasive and metastatic melanoma cells.

Indeed, green tea EGCG-also displayed a significant effect in promote function of let-7b in altering the traits,of malignant cell in melanoma through regulating the expression of HMGA2.with action as DNA transcriptional regulator.

Green tea EGCG. also induced expression of  let-7b  in inhibitions of oncoproteins and activation of tumor suppressor via tumor-over expressed 67LR in activation of PP2A, a tumor suppressor. through adenylate cyclase/cAMP pathway in cell communication.

In compared the effects of TNF-related apoptosis-inducing ligand (TRAIL), a protein with function in in induced tumor cells apoptosis. and green tea bioactive EGCG for treatment of groups of melanoma.

In A375 melanoma cell line, TRAIL application group demonstrated a strong effect in inhibition with apoptosis rate was 11.8% at dose of 150ng/mL in compared to green tea EGCG treatment group of 5%-7% .

Interestingly, in combined TRAIL and green tea EGCG treatment groups, the inhibited rate increased sufficiently to 48.9 - 59.1%

Further analysis also found that EGCG effective in treatment of melanoma was attributed to activity of caspase-3 rather than caspase-8 in mediation of programmed cell death (apoptosis).

The findings suggest that by blocking the sequence of gene expression of tumor, green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) may be considered as an adjunct combined with TRAIL for prevention and treatment of melanoma.

For More information of yoga lessons tailor to a complete well being for women, please visit: YOGA BURN

Arthritis Is Curable
You Can Eliminate Osteoarthritis
By addressing the Underlying Causes through Clinical Trials and Studies

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

FOOD HACK for Weight Loss
A Simple Cooking Technique That Cuts The Calories & Glycemic 
Impact In Rice, Pasta, And Potatoes In Half  

Back to Kyle J. Norton Home page http://kylejnorton.blogspot.ca

Author Biography
Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.

Sources
(1) Epigallocatechin-3-gallate(EGCG) suppresses melanoma cell growth and metastasis by targeting TRAF6 activity by Zhang J1,2, Lei Z1,2, Huang Z3, Zhang X1,2, Zhou Y1,2, Luo Z1,2, Zeng W1,2, Su J1,2, Peng C1,2, Chen X1,2.(PubMed)
(2) EGCG enhances TRAIL-mediated apoptosis in human melanoma A375 cell line by Shen Q1, Tian F, Jiang P, Li Y, Zhang L, Lu J, Li J.(PubMed)
(3) Epigallocatechin-3-O-gallate up-regulates microRNA-let-7b expression by activating 67-kDa laminin receptor signaling in melanoma cells by Yamada S1, Tsukamoto S1, Huang Y1, Makio A1, Kumazoe M1, Yamashita S1, Tachibana H1.(PubMed)

Herbal Therapy: Green Tea polyphenol epigallocatechin-3-gallate (EGCG) in Decreased Progression and Treatment of Her-2/ Positive Breast Cancer

Kyle J. Norton

Green tea may have a strong and positive impact in reduced breast cancer development in women with mutated gene HER2, some scientists suggested.

Breast cancers with HER2 gene amplification or HER2 protein overexpression are called HER2-positive

HER2 is a protein that causes breast cancer cells to grow aggressively.

HER2-positive breast cancers tend to grow faster and are more likely to spread and come back in compared to HER2-negative breast cancers.

Green tea, a precious drink processes numbers of health benefit known to almost everyone in Asia and Western world. However, as yin in nature herbal medicine or food, long term injection of large amounts may obstruct the balance of yin-yang, induced "yin excessive syndrome" or "yang vacuity syndrome" including weaken immunity and painful case of GERD,... according to traditional Chinese medicine's Yin-Yang theory.

Breast cancer is a medical condition characterized by irregular cells growth in the breast tissues. At the late stage, cancerous cell may travel a distance away to infect other organs and tissues from original site.

The study of bioactive green tea polyphenol epigallocatechin-3-gallate (EGCG) was found to decrease progression of breast cancer cells with Her-2/neu over expression, through many mechanisms.

Application of green tea EGCG to Her-2/neu-driven mammary tumor cells showed a significant effect in  blocking over expression of key regulators in the epithelial to mesenchymal transition (EMT) pathway in induced invasive cell proliferation in breast outer layer tissues.

Additionally, the bioactive compound also inhibited Her-2/neu-driven mammary tumor cells by enhanced genes expression in related to tumor suppressor(epithelial genes E-cadherin, gamma-catenin) and promoted tumor cell cycle arrest(MTA3) and involved in the regulation of cellular proliferation and differentiation in target tissues(estrogen receptor alpha (ERalpha)).

Further analysis the study also addressed the important role of green tea EGCG in inhibition of  Her-2/neu-driven mammary tumor cells through decreased the function of snail gene in expression of tumor cells proliferative activity.

In cell migration and invasion assays, green tea EGCG also restrained branching colony growth and invasion of Her-2/neu-driven mammary tumor cells

Interestingly, invasive and metastatic I phenotypes in mouse mammary tumor cells driven by over expression of protein in transmuted transcription of DNA, involved cell survival and a messenger-independent in protect against apoptotic tumor cells also inhibited by administration of green tea EGCG.

Furthermore, green tea EGCG treatment activated FOXO3a in triggered apoptosis of tumor cells and reversed the invasive characteristic of in ERalpha-positive breast cancer cells by blocking expression of protein of transforming growth factor beta in induced tumor cell growth, cell proliferation, cell differentiation.

Further differentiation of over expression of the epidermal growth factor receptor family member Her-2/neu in breast cancer, application of EGCG not only inhibited mouse mammary tumor virus (MMTV)-Her-2/neu NF639 cell growth in culture and soft agar.but also ameliorated risk of basal Her-2/neu receptor tyrosine phosphorylation in contribution of exceptional oncogenic potency. via regulation of enzymes involved in cellular growth, proliferation, differentiation (phosphatidylinositol 3- kinase), and proteins in induced oncogenic transformation (Akt kinase to NF-kappaB pathway).

In SMF derived from mammary gland tumors and cultured and also epidermal growth factor receptor 4,  green tea EGCG inhibited the expression of a significantly higher phosphorylation state of basal receptor which display high constitutive Her-2/neu tumor.

Interestingly in the study of epigallocatechin-3 gallate (EGCG) effects in inhibited growth of Trastuzumab-resistant HER2-driven breast cancer cells, researchers found that  EGCG treatment in a dose-dependent inhibited trastuzumab-resistant BT474 human breast cancer cells, isolated by chronic trastuzumab exposure  and JIMT-1 breast cancer cells, by decreasing tumor growth, tumor cellular ATP production in absorption of chemical energy obtained from the breakdown of food molecules and releases it to fuel other cellular processes, and by inducing tumor cells apoptosis at high concentrations.

Most importantly, EGCG also suppressed multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. of Akt activity, improved FOXO3a function in triggered apoptosis of tumor cells and elevated expression of anti tumor suppressor proteins. p27Kip1

Dr. Eddy SF , the lead author said, "EGCG in combination with trastuzumab may provide a novel strategy for treatment of HER2-over expressing breast cancers, given that EGCG can cross the blood-brain barrier".

Taking altogether, green tea with abundant bioactive polyphenol epigallocatechin-3-gallate (EGCG) may have therapeutic potential in reduced risk and progression of HER2 positive breast cancer, particularly when combined with other chemo-medicine.

Arthritis Is Curable
You Can Eliminate Osteoarthritis
By addressing the Underlying Causes through Clinical Trials and Studies

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

FOOD HACK for Weight Loss
A Simple Cooking Technique That Cuts The Calories & Glycemic
Impact In Rice, Pasta, And Potatoes In Half

Back to Kyle J. Norton Home page http://kylejnorton.blogspot.ca

Author Biography
Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.

Sources
(1) Trastuzumab-resistant HER2-driven breast cancer cells are sensitive to epigallocatechin-3 gallate by Eddy SF1, Kane SE, Sonenshein GE.(PubMed)
(2) Activation of FOXO3a by the green tea polyphenol epigallocatechin-3-gallate induces estrogen receptor alpha expression reversing invasive phenotype of breast cancer cells by Belguise K1, Guo S, Sonenshein GE.(PubMed)
(3) Green tea polyphenol epigallocatechin-3 gallate inhibits Her-2/neu signaling, proliferation, and transformed phenotype of breast cancer cells by Pianetti S1, Guo S, Kavanagh KT, Sonenshein GE.(CANCER RESEARCH 62, 652–655, February 1, 2002])

Herbal Therapy: Green Tea, The Nano-Beverage for Treatment of Ovarian Cancer

Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)

Green tea may have a potential effect in reduced risk and treatment of ovarian cancer, some institute studies suggested

Green tea, a precious drink processes numbers of health benefit known to almost everyone in Asia and Western world. However, as yin in nature herbal medicine or food, long term injection of large amounts may obstruct the balance of yin-yang, induced "yin excessive syndrome" or "yang vacuity syndrome" including weaken immunity and painful case of GERD,... according to traditional Chinese medicine's Yin-Yang theory.

Ovarian cancer is a medical condition characterized by cell growth disorderly in the ovaries. At the late stage, cancerous cells may infect tissues and organs far away from the originated site.

In the analysis of the EGCG effects on 8 ovarian cancer cell lines (SKOV3, CAOV3, OVCAR3, OVCAR10, A2780, CP70, C30, and C200) and showed IC50s for EGCG at the micromolar range, researchers found that EGCG inhibited all ovarian cancer cell lines in dose depending manner.

Normal dose application of EGCG displayed a significant increase of antioxidant in reduced ROS over expression of oxidative stress in inhibited ovarian cancer through cell cycle arrest in G2/M phase.

Further differentiation also revealed that administration of EGCG at common dose not only demonstrated a 6 fold increase of cisplatin potency for treatment of SKOV3, CAOV3, and C200 cells, but also attenuated the chemodrug toxicity.

However, the effect of EGCG on the formation of reactive oxygen species (ROS) was biphasic.
The phytochemical was shown to induce and decrease ROS formation in different doses.

Therefore, higher dose of EGCG promoted over expression of antioxidants in reduced oxidative stress may amplify the toxicity in ameliorated human cancer cells proliferation and induced apoptosis.

Dr., the lead author said, "EGCG may accentuate oxidative stress to inhibit growth of ovarian cancer cells and sensitize them to cisplatin".

Other researchers in the study of toxicity of EGCG suggested that green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG) as a potent adjuvant to enhance the anti tumor efficacy of cisplatin while mitigating its harmful side effects through various mechanisms.

Application of EGCG showed to promote hyaluronic acid function in regulation of normal cell division and prevention of the acid in initiation of cancerous cell proliferation.

Hyaluronic acid, a polysaccharide molecule, plays an important role in cell proliferation and migration, and may involve in the progression of some malignant tumors.

Additionally, EGCG also expressed anti cancer effect by promoting the injection of chemo medicine cisplatin into the CD44-over expressing cancer cells to prevent over growth of tumor cells.

In regard to toxicity, EGCG on one hand, reduced over expression of oxidative stress in initiated toxicity against off-target organs and tissues through induction of a significant antioxidant activity on the hand, and stimulated function of toxicity originating from cisplatin into the target tumors on the other..

Further analysis, green tea catechin-based micellar nanocomplexes also inhibited superiorly against tumor growth with no injection of cisplatin and expressed a significant inhibition without induced toxicity in both cancer cells suspended in culture medium and injected into the animal models and peritoneal metastatic model of human ovarian cancer.

In other words, green tea catechin-based micellar nanocomplexes may be considered as a safe and effective cisplatin nanomedicine for ovarian cancer treatment..

More interestingly, in the additionally examined the effect of EGCG in suppressing ovarian cancer cell growth in 3 human ovarian cancer cell lines (p53 negative, SKOV-3 cells; mutant type p53, OVCAR-3 cells; and wild type p53, PA-1 cells),

EGCG decreased tumor growth in each cell line in a dose-dependent fashion and induced apoptosis and cell cycle arrest, particular to the G(1) phase in SKOV-3 and OVCAR-3 cells in compared to G(1)/S transition phase arrest in PA-1 cells.

The chemical also differentiately upregulated the expression of genes and proteins in promoted cancer cell arrest, suppressed tumor growth and induced cell death (Bax, p21, Retinoblastoma, cyclin D1, CDK4, Bcl-X(L)) by more than 2-fold

Taking altogether, green tea may have a profound effect in reduced risk and treatment of ovarian cancer through expression of phytochemical EGCG. However, intake of green tea extract must be taken with care, as overdoses were found to induce liver toxicity.

For More information of yoga lessons tailor to a complete well being for women, please visit: YOGA BURN



Arthritis Is Curable
You Can Eliminate Osteoarthritis
By addressing the Underlying Causes through Clinical Trials and Studies

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

FOOD HACK for Weight Loss
A Simple Cooking Technique That Cuts The Calories & Glycemic
Impact In Rice, Pasta, And Potatoes In Half

Back to Kyle J. Norton Home page http://kylejnorton.blogspot.ca

Author Biography
Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.

Sources
(1) Hyaluronic acid-green tea catechin micellar nanocomplexes: Fail-safe cisplatin nanomedicine for the treatment of ovarian cancer without off-target toxicity by Bae KH1, Tan S1, Yamashita A1, Ang WX1, Gao SJ1, Wang S1, Chung JE1, Kurisawa M2.(PubMed)
(2) Epigallocatechin-3-gallate delivers hydrogen peroxide to induce death of ovarian cancer cells and enhances their cisplatin susceptibility by Chan MM1, Soprano KJ, Weinstein K, Fong D.(PubMed)
(3) Anticancer effects of (-)-epigallocatechin-3-gallate on ovarian carcinoma cell lines by Huh SW1, Bae SM, Kim YW, Lee JM, Namkoong SE, Lee IP, Kim SH, Kim CK, Ahn WS.(PubMed)

19 Incredible Healthy Benefits of Dried Peas

Kyle J. Norton


Dried pea is a small but nutritionally mighty member of the legume family, genus Pisum belongings to the family Fabaceae with healthy source of proteins, fibers, vitamins and minerals.

1. Dried peas and Types 2 Diabetes
2. Dried peas as Antioxidant
3. Food Therapy - Dried peas and colon cancer
4. Food Therapy - Dried Peas and Weight loss
5. Food Therapy: Dried Peas Fibers In Reduced Risk and Treatment of Hyperlipidemia
6. Food Therapy: Dried Peas and Beans Fibers In Reduced Risk and Treatment of Obesity
7. Food Therapy: Dried Pea and Bean Fibers in Reduced Hypertension Incidence
8. Food Therapy: Dried Peas Water Soluble Fibers in Reduced Risk of Cardiovascular Disease
9. Food Therapy: Dried peas Water Soluble Fibers in Attenuated Risk of Hypercholesterolemia
10. Food Therapy: Dried Peas and Beans In Reduced Risk and Treatment of Colon Cancer(revised)
11. Food Therapy: Dried Peas and Its Water Soluble Fibers In Reduced Risk of Various Forms of Cancer
12. Food Therapy: Dried Peas in Weight Loss and Weight Control
13. Food Therapy: Dried Peas Fiber In Reduced Risk of Constipation and Decreased Laxatives Use
14. Food Therapy: Legumes Dried Peas in Reduced Risk and Treatment of Prostate Cancer
15. Food Therapy: Dried Peas, The Best in Prediction, Prevention and Treatment of Liver Diseases
16. Food Therapy: Common (Dried) Peas In Stimulated Immunomodulating Expression
17. Food Therapy: Dried Peas and Bean Fiber in Attenuated Risk and Treatment of Metabolic Syndrome
18. Food Therapy: Dried Pea, An Antioxidant Functional Food
19. Food therapy: Dried Peas, the Best and Potent Anti inflammatory Agent

Arthritis Is Curable

You Can Eliminate Osteoarthritis

By addressing the Underlying Causes through Clinical Trials and Studies

Ovarian Cysts And PCOS Elimination

Holistic System In Existence That Will Show You How To

Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

FOOD HACK for Weight Loss

A Simple Cooking Technique That Cuts The Calories & Glycemic

Impact In Rice, Pasta, And Potatoes In Half

Back to Kyle J. Norton Home page http://kylejnorton.blogspot.ca

Author Biography
Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.

Food therapy: Green Tea A Potent Beverage in Reduced Risk and Treatment of Osteosarcoma

Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)

Green tea may have a strong implication in reduced risk and treatment of osteosarcoma, some researchers suggested.

Green tea, a precious drink processes numbers of health benefit known to almost everyone in Asia and Western world. However, as yin in nature herbal medicine or food, long term injection of large amounts may obstruct the balance of yin-yang, induced "yin excessive syndrome" or "yang vacuity syndrome" including weaken immunity and painful case of GERD,... according to traditional Chinese medicine's Yin-Yang theory.

Osteosarcoma is a medical condition of bone cancer characterized by producing immature bone and occurred mostly in children and young adults between the ages of 10 and 30.

According to the study in investigation of the effects of Epigallocatechin-3-gallate (EGCG), in reduced risk of osteosarcoma expression in compared to miR-126 in the proliferation, invasion, migration, and chemotherapeutics resistance in cancer, application of Epigallocatechin-3-gallate (EGCG)
1. Exerted a significant effect in suppresses proliferation of human cell line osteosarcoma MG63 and U2OS cells in a concentration-dependent and time-dependent manner
2. Inhibited cancer cell line at the concentration of 0.05 g/L  on U2OS cells were equivalent to 20 μM cisplatin (DDP)

In compared to green tea extract induced cell death by interfering in cell cycle arrest, miR-126, the chemo suppressor drug induced apoptosis and inhibited proliferation in U2OS cell line but without significant effects on phase 1 of cancerous cell cycle division.

Further analysis, researchers suggested application of Epigallocatechin-3-gallate (EGCG) may increase the effect of tumor suppressing drug miR-126 and  for treatment of osteosarcoma. 

In fact, the inhibition of miR-126 osteosarcoma cells without inducing apoptosis and cells proliferation in cell cycle division is attributed to the medicine in target of sequences in reducing the expression of the targeted genes.

In other words,  the medicine can bind directly to inhibit DNA transcription and prevented  mRNA in transmitting such transcription in initiated cancer development. 

Other researchers suggested that the inhibition of Epigallocatechin-3-gallate (EGCG) in reduced cancerous cell death does not limit to the function of cell cycle arrest, the phytochemical compoud also expressed the apoptotic effect through modulating gene expression of cancer cell line and induced degradation of cancerous cellular structure.

Additional evaluation of the phytochemical Epigallocatechin-3-gallate (EGCG) and the tumor suppressed drug miR-1 effect in osteosarcoma, researchers also found that Epigallocatechin-3-gallate has a profound and positive effect in improved the efficacy of miR-1 in induced cell apoptosis and proliferation observed in cancer cell lines MG-63 and U-2OS.

More importantly, MG-63 and U-2OS treated by miR-1 and Epigallocatechin-3-gallate (EGCG) decreased c-MET expression, which is an indication of cancers development.

Further more, combined application of Epigallocatechin-3-gallate (EGCG) and c-MET inhibitor (crizotinib) also displayed a significant inhibition of MG-63 and U-2OS cancer cell proliferation.

More interestingly, in the study of the effects of Se-enriched Ziyang green tea, containing chemical compound mannose, rhamnose and fucose in anti tumor activity on human osteosarcoma U-2 OS cells in vitro and in vivo, researchers found that Se-ZYTP at concentration of 25, 50, 100 and 200 μg/ml exetred a significantly inhibition of proliferation of human osteosarcoma U-2 OS cells in a concentration-dependent fashion.

In cancer cell line In U-2 OS cancer induced in BALB/c athymic mice model, Se-ZYTP oral administration at three doses of 100, 200 and 400mg/kg body weight (B.W.) daily for 28 days resulted in tumor regression as compared to model control without causing any serious side effects.

Once again, we illustrated that other chemical compounds found in green tea may also have the  potential in suppression of  proliferation  and induced apoptosis of osteosarcoma.

In deed, the anti osteosarcoma activities of Se-enriched Ziyang green tea may be attributed to the stimulation in increased pro-apoptotic protein Bax expression(upregulated by the tumor suppressor protein p53 in induced apoptosis) and decreased  expression levels of anti-apoptotic protein Bcl-2(regulator proteins that regulate cell death) and NG2(involved cell survival, migration and angiogenesis), some researchers suggested.

Taking altogether, green tea with plenty of phytochemicals in suppression of the development and progression of cancer may be considered as an adjunct therapy in reduced risk and combination with chemo drugs for treatment of osteosarcoma.

For More information of yoga lessons tailor to a complete well being for women, please visit: YOGA BURN

Arthritis Is Curable
You Can Eliminate Osteoarthritis
By addressing the Underlying Causes through Clinical Trials and Studies

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

FOOD HACK for Weight Loss
A Simple Cooking Technique That Cuts The Calories & Glycemic 
Impact In Rice, Pasta, And Potatoes In Half  

Back to Kyle J. Norton Home page http://kylejnorton.blogspot.ca

Author Biography
Kyle J. Norton (Scholar, Master of Nutrients, All right reserved)
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.

Sources
(1) Overexpression of miR-126 sensitizes osteosarcoma cells to apoptosis induced by epigallocatechin-3-gallate by Jiang L, Tao C, He A, He X1.(PubMed)
92) Green tea polyphenol EGCG suppresses osteosarcoma cell growth through upregulating miR-1 by Zhu K1, Wang W2.(PubMed)
(3) Tumoricidal effects of a selenium (Se)-polysaccharide from Ziyang green tea on human osteosarcoma U-2 OS cells by Wang Y1, Chen J, Zhang D, Zhang Y, Wen Y, Li L, Zheng L.(PubMed)

Food Therapy: Beet or Beet Root, The Best Anti Inflammatory Functional Food

Kyle J. Norton

Beet may be one of function food, having an anti inflammatory potential in reduced pain in damage tissues or local infection.

Inflammation is a natural reaction of immune system in protection of our body during tissues damage, injure and infection

Beet, best known as the beetroot or garden beet and belonging to the amaranth family, is a perennial plant with leafy stems growing to 1–2 m tall.

According to the Meerut Institute of Engineering & Technology, in carrageenan induced paw oedema method for acute inflammation and cotton pellet granuloma for chronic inflammation in rat model, injected aqueous extract of leaves of Beta vulgaris exhibited anti-inflammatory effects at dose level of 1000 mg/kg in comparison to those of indomethacin, a group of nonsteroidal anti-inflammatory drugs (10 mg/kg).

Also in carrageenan (100 µg/paw)-induced paw edema and neutrophil migration to the paw skin tissues.treatment with betalain (100 mg/kg, i.p.) significantly inhibited the expression of paw edema. caused by carrageenan, a common food additive and complete Freund's adjuvant (10 µl/paw) used in animal experiment to induce infection.

Neutrophils are white blood cells formed an important role in defense against infection and inflamamtion caused by injure and microorganisms invasion and neutrophil migration is a rapid movement of white blood cells to the location of infection or inflammation.

According to the study, the anti inflammatory effect of aqueous extract of leaves of Beta vulgaris probably was attributed to phytochemicals found in the extract such as flavonoids, carbohydrates, pentose, amino acids, saponins, tannins and steroid in promoted and increased movement of plasma and leukocytes from the blood into the injured tissues to counteract the invasion of foreign substances and disease.and exhibited the release of histamine in generation of mass and white blood cells and serotonin induced platelets binding to a clot, then ameliorating inflammation through regulation of inflammatory mediators including leukotrienes and prostaglandins,

Please note that

1. Hhormone prostaglandins play a key role in the generation of the inflammatory response and stimulation of constriction and clotting of platelets.

2. Leukotrienes (LTs) is a family of lipid and inflammatory mediators with a function in response to pathogenesis of inflammation

 

The study emphasized that in compared to a group of nonsteroidal anti-inflammatory drugs, aqueous extract of leaves of Beta vulgaris at specific dose exhibited a stronger and higher inhibition of acute inflammation induced by prostaglandin synthesis, including the inflammatory response to diverse stimuli in induced temporary increase of physiological activity.

Further analysis also suggested that in chronic inflammatory diseases induced by cotton pellet granuloma, such as arthritis, aqueous extract of leaves of Beta vulgaris although less effective than the conventional drug of indomethacin, had a profound effect in reduced symptoms and prevented inflammatory process of cells at the site of inflammation by healing of the tissue through the initiated cells shift to induced apoptosis.

In other words, aqueous extract of leaves of Beta vulgaris ameliorated risk of  chronic inflammation by healing the affected tissue, thus preventing cell being damage caused by prolonged inflammation.

Furthermore, application of betalain (100 mg/kg), isolated from beet root also suppressed free radical production and inflammatory activity through enhanced antioxidant activity and inhibited the over expression of proinflammatory proteins such as

TNF-α, a tumor necrosis factor, a protein with function in regulated the production of inflammatory cytokins in the acute phase of invasion by foreign substances, and nterleukin (IL)-1β has a duo functions in activated either pro-inflammatory cytokine or anti-inflammatory myokines.and promoted
 IL-10 expression in production of anti-inflammatory cytokine

In healthy adult, with inflammatory bowel diseases (IBD), betanin, the major component of beet (Beta vulgaris var. rubra) also demonstrated a significant anti inflammatory property in modulated reactive oxygen species (ROS) production and oxidative stress cause of DNA damage as well as increasing apoptosis in neutrophils by analyzing the blood samples of tested subjects.

Dr. Zielińska-Przyjemska M the lead author said, "Betanin treatment at the concentration of 100 μM for 24 h increased DNA damage assessed by comet assay in IBD patients' neutrophils. A similar effect although less pronounced was observed in Caco-2 cells(colon cancer cell line)".

The finding evidences suggested that beet with plenty phytochemicals, may have a therapeutic potential to be utilized in the treatment of inflammation-associated diseases.

Arthritis Is Curable
You Can Eliminate Osteoarthritis
By addressing the Underlying Causes through Clinical Trials and Studies

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

FOOD HACK for Weight Loss
A Simple Cooking Technique That Cuts The Calories & Glycemic
Impact In Rice, Pasta, And Potatoes In Half

Back to Kyle J. Norton Home page http://kylejnorton.blogspot.ca

Author biography
Kyle J. Norton(Scholar, Master of Nutrients), all right reserved.
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.

Sources
(1) Anti-inflammatory activity of aqueous extract of Beta vulgaris L by Jain S1, Garg VK1, Sharma PK1.(PubMed)
(2) Anti-inflammatory activity of betalain-rich dye of Beta vulgaris: effect on edema, leukocyte recruitment, superoxide anion and cytokine production by Martinez RM1, Longhi-Balbinot DT, Zarpelon AC, Staurengo-Ferrari L, Baracat MM, Georgetti SR, Sassonia RC, Verri WA Jr, Casagrande R.(PubMed)
(3) DNA damage and apoptosis in blood neutrophils of inflammatory bowel diseasepatients and in Caco-2 cells in vitro exposed to betanin by Zielińska-Przyjemska M1, Olejnik A2, Dobrowolska-Zachwieja A3, Łuczak M4, Baer-Dubowska W1.(PubMed)

Food therapy: Dried Peas, the Best and Potent Anti inflammatory Agent

Kyle J. Norton


Intake of dried peas daily and regularly may reduce risk and treatment of chronic inflammatory diseases, some institute studies suggested.

Dried pea is a small but nutritionally mighty member of the legume family, genus Pisum belongings to the family Fabaceae with healthy source of proteins, fibers, vitamins and minerals.

Inflammation is a natural process of the immune system to heal body damage and injure caused by invasion of foreign substances, including bacteria and virus.

In the study to evaluate the preventive effects of two pea (Pisum sativum) seed albumin extracts, either in the presence (pea seed extract [PSE]) or absence (albumin fraction from PSE [AF-PSE]) of soluble polysaccharides, in the dextran sodium sulfate (DSS) induced colitis in mice assigned to five groups: one noncolitic and four colitic, researchers found that
1. All treatment groups displayed a significantly intestinal anti-inflammatory effect, observed by reduced microscopic histological damage in comparison with nontreated mice
2. Peas extract treatment group was found to ameliorate pro inflammatory markers through regulating the colonic mRNA expression.

3. The chemical isolated also improve the proteins expression involved in maintaining the epithelial barrier function in prevent the passage of foreign antigens, microorganisms and toxins.

In other words, pea (Pisum sativum) seed albumin extracts not only inhibited auto immune response in expression of RNA in transmitting information to proteins involved production of pro inflammatory cytokins but also strengthened colonic cells in prevented permeation of noxious agents, 

Further differentiation, researchers also indicated that application of peas extract also improved gut bacterial function, partially or totally  in compared to healthy mice.

The findings may provide people with high risk chronic inflammatory diseases to  reconsider adding a portion of dried peas into their daily diet for preventive measure.

Truly, peas extract not only have a profound effect in reduced symptoms implicated by inflammatory cytokins but also hold an important key to restore the gut bacteria partially and totally, particularly in patient with colitis.

Other researchers, in a murine model. study to investigate the effect of enzymatic protein hydrolysates isolated from yellow pea seed in risk of inflammation showed that

1. the isolated protein demonstrated a significant activity in inhibited the secretion of pro-inflammatory cytokines, TNF-α- and IL-6, up to 35 and 80%, respectively
2. Peas proetein also expressed a strong effect in regulate up-regulation of IL-6 secretion by small intestine epithelial cells (IEC), in reduced risk inflammation caused by B-cell terminal differentiation to IgA-secreting cells.

TNF-α, tumor necrosis factor tumor necrosis factor is a protein with function in regulated the production of inflammatory cytokins in the acute phase of invasion by foreign substances.

Interleukin 6 (IL-6) is an interleukin has a duo functions in activated either pro-inflammatory cytokine or anti-inflammatory myokine.

With the amazingly collective information Dr. Ndiaye F, the lead authors suggested, "the resulted peptides could be used as an alternative therapy for the prevention of inflammatory-related diseases".

More importantly in the study to evaluate the anti-inflammatory and platelet aggregation effects of three medicinal plants of Pakistan. Methanolic extract of garden pea, researchers after considering to other con founders postulated that

1.  Methanol extract of Desi chickpea inhibited arachidonic acid (AA) induced platelet aggregation (IC50 value = AA = 46 microg/mL) 

2. In compared to 2 other plants, garden pea (Pisum sativum) demonstrated a strongest effect in inhibited PGE2 by 92% in comparison with untreated control wells), Desi chickpea by 87% and Kabuli chickpea extracts by 65%.

Prostaglandin E2 (PGE-2) is another pro inflammatory protein with function in response to infection or inflammation produced by blood vessel walls.

Arachidonic acid is a polyunsaturated omega-6 fatty acid with function in mediated or modulated inflammatory reactions.

Since all ingredients displayed a significant activity in protect the natural body defense mechanism for the removal of injurious agents, necrosed cells and tissues from the body without elevating the inflammatory production, Dr. Zia-ul-Haq M, the lead author said, " Garden pea, Desi chickpea and Kabuli chickpea could be useful as natural antithrombotic anti-inflammatory materials".

The information collected from above studies, demonstrated that the specific factions or ingredients isolated from peas may have a significant effect in reduced expression of pro inflammatory substances with out taking account of complete picture of the whole food.

Therefore, intake of a large portion dried peas daily of patients with chronic inflammatory diseases does not automatically mean a completely recovered from such illness.

In fact, most studies in the article have used either a small sample size or in a early stage of animal experiment, thus clinical studies are necessary to re confirm these validation.

However, it is safe to suggest that intake of dried peas may have a strong implication in ameliorated risk of inflammation without altering the function of immune modulating effect.

Arthritis Is Curable
You Can Eliminate Osteoarthritis
By addressing the Underlying Causes through Clinical Trials and Studies

Ovarian Cysts And PCOS Elimination
Holistic System In Existence That Will Show You How To
Permanently Eliminate All Types of Ovarian Cysts Within 2 Months

FOOD HACK for Weight Loss
A Simple Cooking Technique That Cuts The Calories & Glycemic
Impact In Rice, Pasta, And Potatoes In Half

Back to Kyle J. Norton Home page http://kylejnorton.blogspot.ca

Author Biography
Kyle J. Norton(Scholar, Master of Nutrients), all right reserved.
Health article writer and researcher; Over 10.000 articles and research papers have been written and published on line, including world wide health, ezine articles, article base, healthblogs, selfgrowth, best before it's news, the karate GB daily, etc.,.
Named TOP 50 MEDICAL ESSAYS FOR ARTISTS & AUTHORS TO READ by Disilgold.com Named 50 of the best health Tweeters Canada - Huffington Post
Nominated for shorty award over last 4 years
Some articles have been used as references in medical research, such as international journal Pharma and Bio science, ISSN 0975-6299.

Sources
(1) Pea (Pisum sativum L.) seed albumin extracts show anti-inflammatory effect in the DSS model of mouse colitis by Utrilla MP1, Peinado MJ, Ruiz R, Rodriguez-Nogales A, Algieri F, Rodriguez-Cabezas ME, Clemente A, Galvez J, Rubio LA.(PubMed)
(2) Anti-oxidant, anti-inflammatory and immunomodulating properties of an enzymatic protein hydrolysate from yellow field pea seeds by Ndiaye F1, Vuong T, Duarte J, Aluko RE, Matar C.(PubMed)
(3) Platelet aggregation and anti-inflammatory effects of garden pea, Desi chickpea and Kabuli chickpea. by Zia-ul-Haq M1, Khan BA, Landa P, Kutil Z, Ahmed S, Qayum M, Ahmad S.(PubMed)